| |
|
|
| Registros recuperados : 17 | |
| 14. |  | HE, Y.; MATHIEU, J.; YANG, Y.; YU, P.; SILVA, M. L. B. da; ALVAREZ, P. J. J. Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. Environmental Science & Technology, v. 4, n. 11, p. 494-499, 2017.| Biblioteca(s): Embrapa Suínos e Aves. |
|    |
| 15. | | SILVA, S. L.; GOMES, R. da C.; BONIN, M. N.; MARTELLO, L. S.; ALVAREZ, P. L.; OLIVEIRA, L. S.; MAZON, M. R.; NOGUEIRA FILHO, J. C. M. Feedlot performance and carcass traits of Nellore cattle as affected by sex condition and frame size. Journal of Animal Science v.90, (Supplement 3/J); Journal of Dairy Science, v.95, (Supplement 2), p.99, 2012. Edição dos trabalhos do:ADSA-PSA-AMPA-ASAS-CSAS-WSASAS JOINT ANNUAL MEETING, 2012, Phoenix-AR. Ruminant Nutrition: Beef I. M266.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 16. | | ALONSO, I. A.; ALFRED ARNOLD, G.; ALONSO ARGÜELLO, G. D.; PRADO, R. B.; CABALLERO ALVAREZ, P. J.; FLORES MONTES, N. G.; MONTEIRO, J. M. G.; MIONI, W. F.; SANJINÉS U. V. E.; TARABORELLI, D. S. Análisis comparado de políticas públicas de protección y gestión de los bosques nativos en el Gran Chaco Sudamericano. In: CONGRESO INTERNACIONAL DEL GRAN CHACO AMERICANO: TERRITORIO E INNOVACIÓN, 3., 2020, Santiago del Estero. El Gran Chaco: buen vivir, diversidad y desarrollo sostenible. Santiago del Estero: INTA, 2020.| Biblioteca(s): Embrapa Solos. |
| |
| 17. | | VEDOVATO, L. B.; CARVALHO, L. C. S.; ARAGÃO, L. E. O. C.; BIRD, M.; PHILLIPS, O. L.; ALVAREZ, P.; BARLOW, J.; BARTHOLOMEW, D. C.; BERENGUER, E.; CASTRO, W.; FERREIRA, J. N.; FRANÇA, F. M.; MALHI, Y.; MARIMON, B.; MARIMON JÚNIOR, B. H.; MONTEAGUDO, A.; OLIVEIRA, E. A.; PEREIRA, L. O.; PONTES-LOPES, A.; QUESADA, C. A.; SILVA, C. V. J.; ESPEJO, J. E. S.; SILVEIRA, M.; FELDPAUSCH, T. R. Ancient fires enhance Amazon forest drought resistance. Frontiers in Forests and Global Change, v. 6, 1024101, 2023.| Biblioteca(s): Embrapa Amazônia Oriental. |
|  |
| Registros recuperados : 17 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Suínos e Aves. |
|
Data corrente: |
18/12/2017 |
|
Data da última atualização: |
18/06/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
HE, Y.; MATHIEU, J.; YANG, Y.; YU, P.; SILVA, M. L. B. da; ALVAREZ, P. J. J. |
|
Afiliação: |
YA HE, Rice University Houston - Texas; JACQUES MATHIEU, Rice University Houston - Texas; YU YANG, Rice University Houston - Texas; PINGFENG YU, Rice University Houston - Texas; MARCIO LUIS BUSI DA SILVA, CNPSA; PEDRO J. J. ALVAREZ, Rice University Houston - Texas. |
|
Título: |
Dioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Environmental Science & Technology, v. 4, n. 11, p. 494-499, 2017. |
|
DOI: |
10.1021/acs.estlett.7b00456 |
|
Idioma: |
Inglês |
|
Conteúdo: |
ABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives. MenosABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites ... Mostrar Tudo |
|
Palavras-Chave: |
Biodegradação de dioxano; Microbactéria. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198674/1/marcio-busi.pdf
|
|
Marc: |
LEADER 02224naa a2200217 a 4500
001 2082755
005 2019-06-18
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1021/acs.estlett.7b00456$2DOI
100 1 $aHE, Y.
245 $aDioxane biodegradation by mycobacterium dioxanotrophicus pH-06 is associated with a group-6 soluble di-iron monooxygenase.$h[electronic resource]
260 $c2017
520 $aABSTRACT: 1,4-Dioxane (dioxane) is a groundwater contaminant of emerging concern for which bioremediation may be a promising strategy. Several bacterial strains can metabolize dioxane or degrade it cometabolically. However, the molecular basis of dioxane biodegradation is only partially understood, and the gene coding for dioxane/ tetrahydrofuran (THF) monooxygenase in Pseudonocardia dioxanivorans CB1190 is the only well-characterized catabolic gene. Here, we identify a novel group-6 propane monooxygenase gene cluster (prmABCD) in Mycobacterium dioxanotrophicus PH-06, which is a bacterium with superior dioxane degradation kinetics compared with CB1190. Whole genome sequencing of PH-06 revealed the existence of a single soluble di-iron monooxygenase (SDIMO). RNA sequencing and reverse transcription quantitative PCR (RT-qPCR) subsequently confirmed that all four components of this gene cluster are upregulated when PH-06 is grown on dioxane compared with growth on acetate or glucose as negative controls. This first characterization of a group-6 SDIMO associated with dioxane biodegradation suggests that dioxane-degrading genes may be more diverse than previously appreciated. A primer/probe set designed to target the large hydroxylase subunit of this gene cluster exhibited high selectivity (no false positives) and high sensitivity (detection limit = 3000−4000 gene copies/mL culture), which may be useful to help assess the presence of dioxane degraders at contaminated sites and minimize false negatives.
653 $aBiodegradação de dioxano
653 $aMicrobactéria
700 1 $aMATHIEU, J.
700 1 $aYANG, Y.
700 1 $aYU, P.
700 1 $aSILVA, M. L. B. da
700 1 $aALVAREZ, P. J. J.
773 $tEnvironmental Science & Technology$gv. 4, n. 11, p. 494-499, 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Suínos e Aves (CNPSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
